Astrocyte-elevated gene-1 overexpression is associated with poor prognosis in gastric cancer
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Xu Jian-bo • Wu Hui • He Yu-long • Zhang Chang-hua • Zhang Long-juan • Cai Shi-rong • Zhan Wen-hua
- چاپ و سال / کشور: 2010
Description
Astrocyte-elevated gene-1 (AEG-1) plays an important role in diverse cancers and its up-regulation is associated with poor survival of patients. However, the status of AEG-1 expression and its significance in gastric cancer are still unclear. In this study, the expression of AEG-1 was studied in different gastric cancer cell lines and gastric cancer tissues. Expression of AEG-1 was significantly higher in gastric cancer tissues than that in normal tissues. Overexpression of AEG-1 was found in 62.9% of gastric cancers and significantly associated with TNM stage and Ki-67 proliferation index (P\0.01). For survival study, overexpression of AEG-1 was significantly associated with poor survival (P\0.01). Further multivariate analysis suggested that AEG-1 overexpression was an independent prognostic factor for the disease. We demonstrated that inhibition of AEG-1 expression by specific siRNA clearly inhibited SGC-7901 cell growth and enhanced cell apoptosis (P\0.01). Inhibition of AEG-1 reduced phosphorylation of AKT and glycogen synthase kinase (GSK)-3b (Ser 9) and decreased the level of b-catenin, lymphoid enhancer binding factor 1 (LEF1), and Cyclin D1. This indicated that AEG-1 may play a role in Wnt/b-catenin-mediated cancer progression. Taken together, overexpression of AEG-1 could be a useful prognostic factor in patients with gastric cancer. Targeted inhibition of AEG-1 may provide a novel therapeutic strategy for gastric cancer.
Med Oncol (2011) 28:455–462 DOI 10.1007/s12032-010-9475-6 Received: 4 February 2010 / Accepted: 25 February 2010 / Published online: 19 March 2010
