Proteomics analysis of MPP+-induced apoptosis in SH-SY5Y cells
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Hongrong Xie • Ming Chang • Xinyu Hu • Danping Wang • Mingxiu Tian • Guoyi Li • Huiyi Jiang • Ying Wang • Zhong Dong • Yuhua Zhang • Linsen Hu
- چاپ و سال / کشور: 2010
Description
Accumulating evidence suggests that oxidative stress plays a pivotal role in dopaminergic neurodegeneration. However, the kinds of proteins involved in the response to oxidative stress remain unclear. In the present study, SH-SY5Y cells were treated with neurotoxin 1-methyl-4-phenyl-pyridinium ion (MPP?) to induce apoptotic neuronal injury. 2D-DIGE followed by MALDITOF- MS was used to determine the changing protein levels. Proteomics analysis revealed that 22 proteins were differentially altered in MPP?-treated SH-SY5Y cells, of which 7 were up-regulated proteins and 15 were downregulated proteins, respectively. Three protein spots were unambiguously identified as sorcin, annexin V, and ribosomal protein P0. The three proteins showed a significant increase in level, suggesting a role in MPP?-induced apoptosis. The functional roles of these three proteins collectively indicate that multiple mechanisms are pertinent in the underlying pathogenesis of Parkinson’s disease (PD), such as apoptosis, calcium homeostasis, and DNA insults.
Neurol Sci (2011) 32:221–228 DOI 10.1007/s10072-010-0340-3 Received: 26 November 2009 / Accepted: 22 May 2010 / Published online: 19 June 2010
