The effect of lentivirus-mediated TH and GDNF genetic engineering mesenchymal stem cells on Parkinson’s disease rat model
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Dezhi Shi • Gong Chen • Li Lv • Lixin Li • Dong Wei • Peiyuan Gu • Juemin Gao • Yi Miao • Weixing Hu
- چاپ و سال / کشور: 2010
Description
This study was designed to assess the potential therapeutic efficacy of gene-modified mesenchymal stem cells (MSCs), MSCs-TH and MSCs-GDNF, in PD rats. Fifty-nine PD rat models were divided into five groups and then the gene-modified MSCs were transplanted into the striatum of rats according to the design. Apomorphineinduced rotational behavior in rats was observed weekly; rats which received both MSCs-TH and MSCs-GDNF showed the most significant improvement compared with those in other groups (P\0.01). Three weeks later, immunohistochemistry analysis found TH-positive cells and GDNF-positive cells in striatal. Eight weeks later, PD rats were killed. HPLC and ELISA results showed DA and GDNF content in striatum of rats which received both MSCs-TH, and MSCs-GDNF was considerably higher compared with those of other groups (P\0.01),respectively. In conclusion, our results suggest that combined transplantation of MSCs expressing TH and GDNF can lead to remarkable therapeutic effects in a rat model of PD.
Neurol Sci (2011) 32:41–51 DOI 10.1007/s10072-010-0385-3 Received: 31 December 2008 / Accepted: 16 July 2010 / Published online: 13 August 2010
