HIF-1a ODD polypeptides increased the expression of HIF1 and VEGF in hypoxic rat cortical neuron
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Xuemin Liu • Juan Du • Qi Xi
- چاپ و سال / کشور: 2011
Description
To investigate the effect of HIF-1a ODD polypeptides on the expression of HIF-1a and VEGF in primary rat cortical neuron in hypoxia. Primary cultured neurons were exposed to hypoxia for 2, 4, and 8 h. Tat- ODD fusion polypeptide was added before hypoxia in the experimental groups. The expression of HIF-1a and VEGF was detected by Western blot and real time PCR. The levels of HIF-1a and VEGF peaked at 4 h then declined in hypoxia. The expression of HIF-1a and VEGF was increased by tat-ODD fusion polypeptide. tat-ODD fusion polypeptide might improve HIF1 adaptation to hypoxia by increased expression of HIF-1a and VEGF.
Neurol Sci DOI 10.1007/s10072-011-0574-8 Received: 7 March 2011 / Accepted: 29 March 2011
