Effects of conjugated linoleic acid on cleavage of amyloid precursor protein via PPARc
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Yi-Chen Li • Qing Chen • Xian-Zi Wan • Xiang-Ling Yang • Xin Liu • Ling Zhong
- چاپ و سال / کشور: 2011
Description
Conjugated linoleic acid (CLA) plays important roles in physiological conditions. The aim of present study was to explore the effects of CLA on the cleavage of amyloid precursor protein (APP) and the potential mechanism involved. The effects of CLA on intracellular APP, BACE1 (b-site APP Cleaving Enzyme1, BACE1), a disintegrin and metalloprotease (ADAM10) and extracellular sAPPa (soluble) were analyzed by RT-PCR, Western blot and ELISA in SH-SY5Y cells. Our study indicated that CLA significantly decreased the expression of BACE1 and increased the extracellular secretion of sAPPa, but not affected the levels of APP and ADAM10. The study also revealed that the nuclear receptor peroxisome proliferators activated receptor c (PPARc) played an important role in the CLA-induced intracellular BACE1 decrease, as well as the extracellular sAPPa increase through knockdown of PPARc transcription using siRNA. We hypothesize that CLA acts as an agonist or ligand, which binds with PPARc and leads to the increase in APP cleavage via a-secretasemediated pathway and the decrease in the deposition of Ab.
Neurol Sci DOI 10.1007/s10072-011-0711-4 Received: 5 January 2011 / Accepted: 13 July 2011
