Diaphragmatic Adaptation Following Intra-abdominal Weight Charging
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Theodossis S. Papavramidis & Efstathios Kotidis & Konstantinos Ioannidis & Angeliki Cheva & Thomai Lazou & George Koliakos & George Karkavelas & Spi
- چاپ و سال / کشور: 2010
Description
Background The diaphragm, the major respiratory muscle, contains three types of muscular fibers in dynamic balance between them. The fiber ratios vary in time in function of conditions, such as aging, hypoproteinemia, exercise, and chronic respiratory load. The diaphragmatic adaptation following abdominal conditions remains an unexplored field. This experimental study aims to identify the changes of the diaphragm due to chronic abdominal weight load. This may find application in conditions such as pregnancy, ascites, visceromegaly, large masses, and morbid obesity. Methods Thirty rabbits were divided into control (A) and study (B) groups. Group B was loaded with weight for 2 months. The left costal hemidiaphragm were stained with H&E and ATPase (fiber typing), while the right underwent biochemical analysis (prooxidative–antioxidative balance, lipid peroxidation, superoxide dismutase, glutathione reductase and peroxidase activities, total glutathione, and protein carbonylation). Results In H&E, all fibers were within normal range. ATPase analysis demonstrated reduction of type I (p= 0.019) and an increase of the type ةةء fibers ratio (p<0.001) in group B, while the type ةةآ/X fibers ratio remained stable. The above suggest remodeling of type I fibers into type IIA. Concerning biochemical analysis, difference was observed in glutathione peroxidase activity (p<0.001). Conclusions Chronically loaded abdomen leads to morphological adaptations of the costal diaphragm, but with minor oxidative stress. These diaphragmatic morphological changes are equivalent to heart failure or severe COPD, showing that this remodeling makes the muscle more efficient towards work load, but more vulnerable to fatigue.
OBES SURG DOI 10.1007/s11695-010-0334-5
