Modeling the protein partitioning in aqueous polymer two-phase systems: in(uence of polymer concentration and molecular weight
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Dong-Qiang Lin, You-Ting Wu, Le-He Mei, Zi-Qiang Zhu, Shan-Jing Yao
- چاپ و سال / کشور: 2003
Description
Aqueous polymer two-phase system provides a powerful method for separation and puri:cation of biomaterials. Among various factors, polymer concentration and polymer molecular weight are essential and have strong impact on the protein partitioning in these systems. Based on the modi:ed Pitzer’s model, a simple expression has been obtained for correlating protein partitioning in aqueous polymer two-phase systems with varying polymer concentration and di@erent polymer molecular weights. Using only one group of parameters for each target protein, the partition coeBcients of :ve proteins (lysozyme, chymotrypsinogen-A, bovine serum albumin, transferrin and catalase) in 16 sets of polyethylene glycol (PEG)/dextran systems were correlated. In addition, partition behavior of lactate dehydrogenase in PEG/hydroxypropyl starch systems were measured, correlated and partially predicted. A comparison of calculated and experimental data indicated that the model provides good correlation and prediction abilities on the protein partitioning in aqueous polymer two-phase systems with a wide range of polymer concentration and molecular weight.
Chemical Engineering Science 58 (2003) 2963 – 2972 Received 6 May 2002; received in revised form 15 October 2002; accepted 26 March 2003
