High-cell-density cultivation of microorganisms
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : D. Riesenberg ل R. Guthke
- چاپ و سال / کشور: 1999
Description
High-cell-density cultivation (HCDC) is re- quired to improve microbial biomass and product for- mation substantially. An overview of HCDC is given for microorganisms including bacteria, archae and eukarya (yeasts). Problems encountered by HCDC and their possible solutions are discussed. Improvements of strains, di€erent types of bioreactors and cultivation strategies for successful HCDC are described. Stirred- tank reactors with and without cell retention, a dialysis- membrane reactor, a gas-lift reactor and a membrane cyclone reactor used for HCDC are outlined. Recently modiRed traditional feeding strategies and new ones are included, in particular those for unlimited growth to very dense cultures. Emphasis is placed on robust fer- mentation control because of the growing industrial interest in this Reld. Therefore, developments in the application of multivariate statistical control, artiRcial neural networks, fuzzy control and knowledge-based supervision (expert systems) are summarized. Recent advances using Escherichia coli پ} the pioneer organism for HCDC پ} are outlined.
Appl Microbiol Biotechnol (1999) 51: 422±430 Received: 20 October 1998 / Received revision: 18 December 1998 / Accepted: 21 December 1998
