Infuence of acetic acid on the growth of Escherichia coli K12 during high-cell-density cultivation in a dialysis reactor
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : K. Nakano ل M. Rischke ل S. Sato ل H. Maب rkl
- چاپ و سال / کشور: 1997
Description
High-cell-density cultivations of Escherichia coli K12 in a dialysis reactor with controlled levels of dissolved oxygen were carried out with di€erent carbon sources: glucose and glycerol. Extremely high cell con- centrations of 190 g/l and 180 g/l dry cell weight were obtained in glucose medium and in glycerol medium respectively. Di€erent behaviour was observed in the formation of acetic acid in these cultivations. In glucose medium, acetic acid was formed during the earlier phase of cultivation. However, in glycerol medium, acetic acid formation started later and was particularly rapid at the end of the cultivation. In order to estimate the inپPuence of acetic acid during these high-cell-density cultivations, the inhibitory e€ect of acetic acid on cell growth was investigated under di€erent culture conditions. It was found that the inhibition of cell growth by acetic acid in the fermentor was much less than that in a shaker cul- ture. On the basis of the results obtained in these in- vestigations of the inhibitory e€ect of acetic acid, and the mathematical predictions of cell growth in a dialysis reactor, the inپPuence of acetic acid on high-cell-density cultivation is discussed.
Appl Microbiol Biotechnol (1997) 48: 597±601 Received: 20 May 1997 / Received revision: 12 August 1997 / Accepted: 25 August 1997
