Gene transfer: the challenge of regulated gene expression
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Z. Sheng Guo1, Qiang Li1, David L. Bartlett1, James Y. Yang2 and Bingliang Fang3
- چاپ و سال / کشور: 2008
Description
Gene therapy is expected to have a major impact on human healthcare in the future. However, precise regulation of therapeutic gene expression in vivo is still a challenge. Natural and synthetic enhancer–promoters (EPs) can be utilized to drive gene transcription in a temporal, spatial or environmental signal-inducible manner in response to heat shock, hypoxia, radiation, chemotherapy, epigenetic agents or viral infection. To allow tightly regulated expression, a regulatable geneexpression system can also be implemented. Most of these systems are based on small molecule (drug)- responsive artificial transactivators. In this review, we aim to provide a brief overview of the classes of EPs and regulatable systems, along with lessons learned from these studies. We highlight the potential applications in gene transfer, gene therapy for cancer and genetic disease and the future challenges for clinical applications.
Corresponding author: Guo, Z.S. (guozs@upmc.edu). 410 1471-4914/$ – see front matter 2008 Elsevier Ltd. All rights reserved. doi:10.1016/j.molmed.2008.07.003 Available online 7 August 2008
