The Development of Chromosome Microdissection and Microcloning Technique and its Applications in Genomic Research
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Ruo-Nan Zhou1, 2 and Zan-Min Hu1,*
- چاپ و سال / کشور: 2007
Description
The technique of chromosome microdissection and microcloning has been developed for more than 20 years. As a bridge between cytogenetics and molecular genetics, it leads to a number of applications: chromosome painting probe isolation, genetic linkage map and physical map construction, and expressed sequence tags generation. During those 20 years, this technique has not only been benefited from other technological advances but also cross-fertilized with other techniques. Today, it becomes a practicality with extensive uses. The purpose of this article is to review the development of this technique and its application in the field of genomic research. Moreover, a new method of generating ESTs of specific chromosomes developed by our lab is introduced. By using this method, the technique of chromosome microdissection and microcloning would be more valuable in the advancement of genomic research.
Current Genomics, 2007, 8, 67-72 Received on: October 20, 2006 - Revised on: November 23, 2006 - Accepted on: November 28, 2006
