Cautionary Tail: The Presence of an N-Terminal Tag on Dynein Light- Chain Roadblock/LC7 Affects Its Interaction with a Functional Partner
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Jikui Song and John L. Markley*
- چاپ و سال / کشور: 2007
Description
As part of structural investigations of components of the molecular motor, dynein, we prepared the light chain, Robl1_mouse, with and without an N-terminal His-tag. We found that the His-tag introduced a spurious binding site for a second protein, IC74. We propose a molecular mechanism for functional interference by the His-tag.
Protein & Peptide Letters, 2007, 14, 265-268