TWIST1 Gene: First Insights in Felis catus
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Clلudia S. Baptista1,2, Estela Bastos2, Sara Santos2, Ivo G. Gut3, Henrique Guedes-Pinto2, Fلtima Gنrtner4,5 and Raquel Chaves*,2
- چاپ و سال / کشور: 2010
Description
TWIST1 is thought to be a novel oncogene. Understanding the molecular mechanisms regulating the TWIST1 gene expression profiles in tumor cells may give new insights regarding prognostic factors and novel therapeutic targets in veterinary oncology. In the present study we partially isolated the TWIST1 gene in Felis catus and performed comparative studies. Several primer combinations were used based on the alignments of homologous DNA sequences. After PCR amplification, three bands were obtained, purified and sequenced. Several bioinformatic tools were utilized to carry out the comparative studies. Higher similarity was found between the isolated TWIST1 gene in Felis catus and Homo sapiens (86%) than between Homo sapiens and Rattus norvegicus or Mus musculus (75%). Partial amino acid sequence showed no change in the four species analyzed. This confirmed that coding sequences presented high similarity (~96%) between man and cat. These results give the first insights regarding the TWIST1 gene in cat but further studies are required in order to establish, or not, its role in tumor formation and progression in veterinary oncology.
Current Genomics, 2010, 11, 212-220 Received on: December 17, 2009 - Revised on: February 22, 2010 - Accepted on: February 24, 2010
