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The MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] Assay Is a Fast and Reliable Method for Colorimetric Determination of Fungal Cell Densities
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : FLORIAN M. FREIMOSER, CLAUDE A. JAKOB,† MARKUS AEBI, AND URS TUOR*
- چاپ و سال / کشور: 1999
Description
The entomopathogenic fungus Neozygites parvispora (Entomophthorales: Zygomycetes) grows in vitro as irregularly rod-shaped hyphal bodies in a complex medium. In order to simplify the medium composition and determine growth-promoting compounds for the cultivation of this fungus, we were looking for a rapid and quantitative method to estimate the number of living cells in small volumes of liquid culture. A colorimetric method for the determination of cell densities using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] proved to be more accurate and timesaving than conventional hemocytometer counting
APPLIED AND ENVIRONMENTAL MICROBIOLOGY,Vol. 65, No. 8,Received 15 January 1999/Accepted 8 May 1999
