High Stability of Recombinant Proteins Expressed in Tobacco Chloroplasts
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Andrea Molina and Jon Veramendi*
- چاپ و سال / کشور: 2009
Description
The green fluorescent protein and the non-toxic subunit of cholera toxin were expressed in tobacco chloroplasts. Both recombinant proteins accumulated to levels greater than 20% of the total soluble protein. We did not observe light-dependent induction of gene expression despite employing the promoter and 5´-UTR from the psbA gene. Both proteins were stable in young and old leaves. The estimated half-life, determined by pulse-chase labeling, was greater than 48 h. Freeze-drying of pulverized leaves supplied an easy method of post-harvest handling with no significant loss of the recombinant protein after 7 months of storage at room temperature. These data suggest that both proteins are good candidates for the expression of fusion proteins (e.g. with antigens) in tobacco chloroplasts.
The Open Biotechnology Journal, 2009, 3, 67-72
