New Emergent Nanotechnologies in Medical and Biochemical Applications: Advanced Fluorescence Protein-Based Nanosensors
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Maria Staiano1, Marcella de Champdoré1, Stefano Borini2, Andrea M. Rossi2, Mosè Rossi1 and Sabato D’Auria*,1
- چاپ و سال / کشور: 2007
Description
In this review we explore the advantages deriving from the use of either enzymes or binding proteins to develop non-consuming substrate fluorescence biosensors. We report on a novel approach to address the consumption of substrate by enzyme-based biosensors, namely the utilization of apo-enzymes as non-active forms of proteins, which through still able to bind the ligand yet cannot transform it into product. We also report recent studies in which fluorescence labelling of a naturally thermostable binding protein by a fluorescent probe allows a quantitative monitoring of glucose. Finally, we will illustrate a novel methodology based on the utilization of porous silicon chips that allows a nanotechnological approach to the realization of protein arrays for analyses of high medical and biochemical interest.
Current Chemcial Biology, 2007, 1, 3-9
