Betaine improves the PCR amplification of GC-rich DNA sequences
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Wolfgang Henke*, Kerstin Herdel, Klaus Jung, Dietmar Schnorr and Stefan A. Loening
- چاپ و سال / کشور: 1997
Description
Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.
1997 Oxford University Press Nucleic Acids Research, 1997, Vol. 25, No. 19 3957–3958 Received May 14, 1997; Revised and Accepted August 5, 1997
