hTERT gene inhibition studies in cancer cells by using polysaccharide lentina
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : k. Sreenivasulu1*, M. Vijayalakshmi2 and k. Sambasivarao3
- چاپ و سال / کشور: 2011
Description
Lentinan a polysaccharide from medicinal mushroom that is Lentinus has been known to have anticancer properties. Telomerase activity is not observed in normal healthy cells, where as in cancerous cells telomerase expression is high. Telomerase represents a promising cancer therapeutic target. We investigated the inhibitory effect of lentinan on telomerase reverse transcriptase gene (hTERT) which is essential for telomerase activity. To assess the transcriptional effect, DLD -1 cancer cells were cultured in the presence of various concentrations of lentinan. TRAP assay, RT-PCR analysis were performed to find telomerase activity and hTERT gene expression respectively. Since c-MYC is known to regulate hTERT, expression of c-MYC was also determined. Culturing cells with lentinan resulted in down regulation of hTERT and c-myc expression. These results indicate that lentinan inhibits telomerase activity by down regulating hTERT expression via suppression of c-MYC in cancer cells.
Journal of Medical Genetics and Genomics Vol. 3(1), pp. 7 – 12, January, 2011 Available online http://www.academicjournals.org/jmgg ISSN 2141-2278 ©2011 Academic Journals Accepted 22 December, 2010
