Effect of platelet activating factor and butyrate on the expression of interleukin-2 receptor · in nasopharyngeal carcinoma cells
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : MING-HSUI TSAI1, SHIOW-HER CHIOU3 and KUAN-CHIH CHOW2
- چاپ و سال / کشور: 2001
Description
Serum level of soluble interleukin-2 receptor · (sIL-2R·) has been shown to correlate with disease progression and prognosis of cancer patients. However, the available information about the source and the pathophysiological regulation of IL-2R· in cancer cells is limited. This study addressed the questions of prognostic value and the source of sIL-2R· in patients with nasopharyngeal carcinoma (NPC). Biological regulation of IL-2R· was characterized in NPC cell lines. Serum sIL-2R· levels of 113 NPC patients were measured by enzyme-linked immunosorbent assay (ELISA). Levels of sIL-2R· in NPC patients were significantly higher than that in the healthy controls, and sIL-2R· levels were correlated with disease progression and patient survival. IL-2R· was identified in cancer cells by immunocytochemistry. In vitro, IL-2R· expression was markedly increased following treatment with platelet activating factor and/or n-sodium butyrate. Increased secretion of IL-2R· was also detected in the culture media. The secreted IL-2R· could functionally bind IL-2. These results indicate that elevated sIL-2R· was often detected in patients with advanced NPC. The elevated sIL-2R· could be shed from NPC cells by a yet to be determined mechanism and IL-2R· expression in NPC cells could be upregulated by platelet activating factor and butyrate.
INTERNATIONAL JOURNAL OF ONCOLOGY 19: 1049-1055, 2001 Received August 10, 2001; Accepted September 11, 2001
