Expression of an antibody-targeted plasminogen activator in Escherichia coli using chemically induced stress responses
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Ming Yu, Meimin Yu & Binggen Ru
- چاپ و سال / کشور: 2004
Description
A recombinant gene coding for an antibody-targeted urokinase-type plasminogen activator was constructed for the purpose of enhancing the thrombolytic specificity of urokinase. The recombinant gene was cloned into prokaryotic expression vector pTrcHisA, and transformed into Escherichia coli strain Rosetta (DE3). Less than 4 mg of the desired protein l)1 could be obtained in the form of inclusion bodies. Of various inducers and enhancers of stress responses, the heat-shock enhancer, streptomycin, the osmotic stress inducers, D-arabinose and sucrose, and the cold-shock enhancer, tetracycline, simulated the expression of the antibody-targeted plasminogen activator by 2–5-fold.
Biotechnology Letters 26: 1629–1634, 2004.
