Modeling and simulation of protein adsorption in permeable chromatographic packings: a double linear driving force model
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Anabela Leitaؤo, Alُآrio Rodrigues1,*
- چاپ و سال / کشور: 1999
Description
Permeable large-pore packings have many applications, particularly in perfusion chromatography for bioseparations. The major objectives of this paper are: (i) to develop a double linear driving force (LDF) model based on linear driving force approximations inside throughpores and microparticles to simulate dynamic nonlinear adsorption in columns having spherical permeable chromatographic packings with a bidisperse pore structure; and (ii) to analyze the performance of this simple model to simulate breakthrough and elution curves for bovine serum albumin adsorption on POROS Q/M large-pore particles (PerSeptive Biosystems, Cambridge, MA, USA), in comparison with a more realistic and dif®cult model (intraparticle diffusion/convection model) which is also presented in this paper. Model results obtained for several values of the intraparticle Peclet number, , resulting from the convective ¯ow in the throughpores show the very reasonable performance of the double LDF model in simulating breakthrough and elution curves of proteins, particularly for higher values of . This ®nding is of relevance due to the remarkable saving in computation time afforded with the double LDF model. # 1999 Elsevier Science S.A. All rights reserved.
Biochemical Engineering Journal 3 (1999) 131-139 Received 12 June 1998; accepted 20 January 1999
