Proteome Analysis to Assess Physiological Changes in Escherichia coli Grown Under Glucose-Limited Fed-Batch Conditions
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Babu Raman, M.P. Nandakumar, Vignesh Muthuvijayan, Mark R. Marten
- چاپ و سال / کشور: 2005
Description
Proteome analysis was used to compare global protein expression changes in Escherichia coli fermentation between exponential and glucose-limited fed-batch phase. Two-dimensional gel electrophoresis and MALDITOF mass spectrometry were used to separate and identify 49 proteins showing >2-fold difference in expression. Proteins upregulated during exponential phase include ribonucleotide biosynthesis enzymes and ribosomal recycling factor. Proteins upregulated during fedbatch phase include those involved in high-affinity glucose uptake, transport and degradation of alternate carbon sources and TCA cycle, suggesting an enhanced role of the cycle under glucose- and energy-limited conditions.Wereport the upregulation of several putative proteins (ytfQ, ygiS, ynaF, yggX, yfeX), not identified in any previous study under carbon-limited conditions.
BIOTECHNOLOGY AND BIOENGINEERING, VOL. 92, NO. 3, NOVEMBER 5, 2005 Received 1 September 2004; accepted 15 March 2005
