Cell and process design for tar ...
This paper is a review of strategies to introduce protein into the liquid medium of Escherichia coli K-12 industrial production cells. The cell desi ...
A DNA vaccine against foot-and- ...
A plasmid DNA vaccine candidate (pCEIS) encoding two foot-and-mouth disease virus (FMDV) VP1 epitopes (amino acid residues 141–160 and 200–213) has b ...
Effects of acrylic acid on prep ...
Dextran hydrogels were obtained by radical copolymerization of methacrylated dextran (MA-dextran) with acrylic acid (AAc) using ammonium peroxydisulf ...
B-Casein Bilayer Adsorption at ...
Ellipsometric and surface pressure studies of â-casein adsorption layers at the water/air interface support the idea of a model that assumes the for ...
Selection of Highly Productive ...
In mammalian cell culture, the selection of high producers is a critical step in efficient recombinant protein production. Drug-resistance selection ...
Novel Escherichia coli Strain A ...
An important characteristic of promoters used in recombinant protein production in Escherichi coli is their inducibility in a simple and cost-effect ...
State of the art in the deliver ...
In photodynamic therapy, one of the problems limiting the use of many photosensitizers (PS) is the difficulty in preparing pharmaceutical formulation ...
High-performance tangeptial flo ...
High-performance tangential flow filtration (HPTFF) is a new technology for protein and nucleotide purification. Conventional ultrafiltration (UF) is ...
Metabolic Load of Recombinant P ...
The strong expression of recombinant proteins in bacteria affects the primary carbon and energy metabolism resulting in growth inhibition and acetat ...
Metabolic Adaptation of Escheri ...
The metabolic burden and the stress load resulting from temperature-induced production of human basic fibroblast growth factor is connected to an in ...
Limiting Factors in Escherichia ...
Fed-batch production of recombinant -galactosidase in E. coli was studied with respect to the specific growth rate at induction. The cultivations w ...
Characterization of enthalpic e ...
Protein adsorption can be either endothermic or exothermic depending upon the protein, the sorbent and process conditions. In the case of protein ads ...
Isothermal titration microcalor ...
The effect of (NH4)2SO4 concentrations on the interaction mechanism between myoglobin and two hydrophobic adsorbents, butyl- and octyl-Sepharose was ...
Preparative liquid chromatograp ...
The status of the theory and the main methods of implementation of preparative liquid chromatography are reviewed. On the theory front, the focus has ...
A theoretical analysis of the t ...
Although the denaturing of adsorbed proteins on biomaterials surfaces is believed to lead to adverse tissue reactions to implanted materials, very li ...
Interaction of Bovine Myelin Ba ...
Despite the essential role played by myelin basic protein (MBP) in stabilizing the multilamellar structure of the myelin membrane, attempts at decip ...
Effects of acrylic acid on prep ...
Dextran hydrogels were obtained by radical copolymerization of methacrylated dextran (MA-dextran) with acrylic acid (AAc) using ammonium peroxydisulf ...
Sustained delivery of human gro ...
Purpose: The purpose of this study was to evaluate the release of recombinant human growth hormone (rhGH) from a novel nonpolymeric sustained release ...
Biodegradable laminar implants ...
Due to its short half-life, renal toxicity and necessity for daily subcutaneous injections, recombinant human growth hormone (rhGH) would best be adm ...
Effect of Static Pressure on Hu ...
The effect of static pressure on the production of human granulocyte-macrophage colony stimulating factor (hGM-CSF) by the Chinese hamster ovary (CHO ...
Toward Elucidating the Full Spe ...
Our research has focused on the molecular design of immunotherapeutic vaccines and the advancement of mite-allergy diagnosis. Here, we describe the r ...
Increased production and recove ...
Although plant cell cultures can be used to produce a variety of proteins, the instability and degradation of secreted proteins hinders product recov ...
Osteopontin regulates anabolic ...
Summary In this pilot study, we demonstrated that women with osteopontin (OPN) over-expression show less resistance to postmenopausal osteoporosis t ...
Reconstruction of Amputated Thu ...
Objective Restoration and/or improvement of pinch and grasp through elongation of the first metacarpal and deepening of the first web space. Indic ...
llioinguinal Approach for Aceta ...
Objective Exposure of the entire anterior column of the acetabulum and of the inner part of the posterior column. Indications Open reduction and i ...