Crystallographic methods and pr ...

Overexpression, isolation, and crystallization of proteins / Jane V. Skelly, C. Bernadette Madden -- Preliminary characterization of crystals / Sheri ...

Basic protein and peptide proto ...

Baculovirus expression protocol ...

Introduction to the molecular biology of baculoviruses / Jorge E. Vialard, Basil M. Arif, Christopher D. Richardson -- Baculovirus transfer vectors / ...

Peptide synthesis protocols

Procedures to improve difficult couplings / Michael W. Pennington, Michael E. Byrnes -- Methods for removing the Fmoc group / Gregg B. Fields -- Sol ...

Peptide analysis protocols

Gel-filtration chromotography / Daniel M. Bollag -- Ion-exchange chromatography / Daniel M. Bollag -- Reversed-phase HPLC : analytical procedure / U ...

Antibody engineering protocols

SEQHUNT : a program to screen aligned nucleotide and amino acid sequences / George Johnson, Tai Te Wu, Elvin A. Kabat -- Molecular modeling of antibo ...

ELISA : theory and practice

Basic immunology -- Basic principles of ELISA -- Stages in ELISA -- Theoretical considerations -- Direct ELISA : titration of antigen and antibody ...

In situ hybridization protocols

Preparation of human chromosomal painting probes from somatic cell hybrids / Nicoletta Archidiacono, Rachele Antonacci, Antonino Forabosco, Mariano Ro ...

In vitro toxicity testing proto ...

Alternative method of assessing toxicity / Chris K. Atterwill -- LLC-RK1 cell screening test for nephrotoxicity / David J. White, Chris Seaman -- Pr ...

In vitro transcription and tran ...

Transcription in vitro using bacteriophage RNA polymerases / Elaine T. Schenborn -- Subtraction hybridization cDNA libraries / Clifford W. Schweinfes ...

Cryopreservation and freeze-dry ...

Virus cryopreservation and storage / Ernest A. Gould -- Freeze-drying and cryopreservation of bacteria / Stephen F. Perry -- Freeze-drying of yeasts ...

Diagnostic bacteriology protoco ...

Antibiotic resistance profiling / Stephen J. Hammonds -- Bacteriocin typing / Tyrone L. Pitt, Michael A. Gaston -- Bacteriophage typing / Tyrone L. ...

Signal transduction protocols

The problems and pitfalls of radioligand binding / Mary Keen -- Solubilization and purification of a functional ionotropic excitatory amino acid rece ...

Protein stability and folding : ...

Noncovalent forces important to the conformational stability of protein structures / Kenneth P. Murphy -- Degradative covalent reactions important to ...

Agrobacterium protocols

Growth and storage of Agrobacterium / Fraukje M. Asma -- Simple cultural tests for identification of Agrobacterium biovars / Hacène Bouzar, Jeffrey ...

Animal cell electroporation and ...

Electroporation theory : concepts and mechanisms / James C. Weaver -- Effects of pulse length and strength on electroporation efficiency / Sek Wen Hu ...

Monoclonal antibody protocols

Methods of immunization to enhance the immune response to specific antigens in vivo in preparation for fusions yielding monoclonal antibodies / Jon A. ...

Protein NMR techniques

Introduction to the NMR of proteins -- Double and triple resonance NMR methods for protein assignment -- Protein chemical shifts -- Data processing ...

Electroporation protocols for m ...

Electroporation theory : concepts and mechanisms / James C. Weaver -- Instrumentation / Gunter A. Hofmann -- Direct plasmid transfer between bacteri ...

YAC protocols

Generation of large insert YAC libraries / Zoia Larin, Anthony P. Monaco, Hans Lehrach -- YAC library storage and transport / John E. Collins, Sheila ...

Plant gene transfer and express ...

Tools for expressing foreign genes in plants / François Guerineau -- Introduction of cloning plasmids into Agrobacterium tumefaciens / Charles H. Sh ...

Species diagnostics protocols : ...

Isolation and purification of plant nucleic acids : genomic and chloroplast DNA / Mien T. Ven, Patrick G. Lanham, Rex M. Brennan -- Isolation and pur ...

Plant cell electroporation and ...

Electroporation theory : concepts and mechanisms / James C. Weaver -- Effects of pulse length and strength on electroporation efficiency / Sek Wen Hu ...

In vitro mutagenesis protocols

Site-directed mutagenesis using positive antibiotic selection / Richard N. Bohnsack -- In vitro site-directed mutagenesis using the unique restrictio ...

Recombinant gene expression pro ...

Recombinant gene expression protocols : overview of experimental strategies for the expression of recombinant genes / Rocky S. Tuan -- Designing expr ...